LC-MS/MS – Steroids
LC-MS/MS – Steroids
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Measurands currently available include: Cortisol and Testosterone. A Progesterone method is currently awaiting validation and Oestradiol will be developed shortly.
Traceability - Serum/Plasma (urine - cortisol)
| Measurand | Standard (certfied purity) | Control Material |
|---|---|---|
| Oestradiol | NMIS CRM 6004-9 | SRM 576, 577, 578 |
| Progesterone | NMIS CRM 6003-9 | SRM 347, 348 |
| Testosterone | M914 (AGAL) >99% | NIST 971 |
| Cortisol | SRM 921 | SRM 192, 193 |
Measurement Method Details
| Measurand / Internal Standard | Sample pre-treatment | Transitions | |||
|---|---|---|---|---|---|
| Steroid Parent | Steroid Daughter | Internal Standard Parent | Internal Standard Daughter |
||
| Cortisol [2,3,4 - 13C3] Cortisol | Liquid-liquid extraction (methyl tertiary butyl ether) | 363.3 | 121.2, 97.15 | 366.3 | 124.2, 100.15 |
| Testosterone [2,3,4 - 13C3] Testosterone | Liquid-liquid extraction (ethyl acetate / hexane) | 289.0 | 97.0, 109.0 | 292.1 | 100.0, 112.0 |
| Progesterone [2,3 - 13C2] Progesterone | Liquid-liquid extraction (hexane) | 315.1 | 97.1, 109.0 | 317.2 | 99.0, 111.0 |
Accreditation
